Mouse aortic smooth muscle cells

Susan Z. Williams

May 19, 2025

8 min read

Mus musculus, mouse.Vascular smooth muscle cells (VSMCs) play important roles in cardiovascular disorders and biology. Figure 1 shows a representative example of measurements performed . Reproducibility of Results. Vascular smooth muscle cells (VSMCs) and extracellular matrix (ECM) maintain integrity of the aortic wall. Here we show the adaptation of this method to isolate and culture mouse aortic VSMCs. The mouse is a critical model for research, however, the isolation of primary .Pathogenesis of aortic aneurysms.Auteur : Neeta Adhikari, Kadambari Chandra Shekar, Rodney Staggs, Zaw Win, Kerianne Steucke, Yi Wei Lin, Li N. Collectively, our .

Smooth Muscle Cell Reprogramming in Aortic Aneurysms

VSMCs were incubated at 37 °C in 5 % CO 2 with MEM supplemented with 1 mM l-glutamine, 100 IU/mL penicillin, 100 μg/mL streptomycin, and 10 % FBS. Neoplastic cells, .

Isolation of vascular smooth muscle cells from a single murine aorta

Vascular smooth muscle cells (VSMCs) have been cultured for decades to study the role of these cells in cardiovascular disorders. 1d–g) confirmed PDE4D depletion in mouse aortic SMCs. Our studies indicated that mouse aortic VSMCs cultured in three-dimensional spheroids acquire a .The results showed that smooth muscle NCOR1 deficiency increased the incidence of aortic aneurysms and exacerbated medial degeneration in angiotensin II-induced AA mouse model. Microscopy, Fluorescence. Transdifferentiation of mouse aortic smooth muscle cells to a macrophage-like state after cholesterol loading. spindle-shaped. The most common source of VSMCs is the rat aorta.Vascular smooth muscle cells (VSMCs) play a key role in aortic aneurysm formation.Given that atherosclerosis complications are the main cause of death in progeria, here, we investigated whether progerin-induced atherosclerosis is prevented in . However, the phenotype of modulated .

Bioengineering

The healthy aorta (left) consists of intimal, medial, and adventitial layers.In response to various stimuli, vascular smooth muscle cells (SMCs) can de-differentiate, proliferate and migrate in a process known as phenotypic modulation.In a Marfan syndrome mouse model (Fbn1 C1039G /+), single‐cell RNA sequencing combined with an SMC lineage tracing study has shown a specific SMC population in the diseased aorta marked by .

The physiology of vascular smooth muscle (VSMC) cells is affected by autophagy, a catabolic cellular mechanism responsible for nutrient recycling.Objective: We previously showed that cholesterol loading in vitro converts mouse aortic vascular smooth muscle cells (VSMC) from a contractile state to one resembling macrophages.Abrogation of TGF-β signaling in smooth muscle cells (SMCs) in combination with hypercholesterolemia results in formation of aortic aneurysms due to reprograming of a subset of normal SMCs into mesenchymal-like stem cells that give rise to adipocytes, chondrocytes, and osteoblasts, as well as into macrophage-like cells.

Increasing pulse pressure ex vivo, mimicking acute physical

We also observed no . Proc Natl Acad Sci U S A. Vascular smooth muscle cells (vSMC) are .Mouse aortic smooth muscle cells (SMCs) were loaded for 72 h with cholesterol by using cholesterol:methyl-β-cyclodextrin complexes, leading to ≈2-fold and .

( A-E ) In the proprotein convertase subtilisin/kexin type 9 (PCSK9)/angiotensin II (AngII) model, after inducing hyperlipidemia by AAV-PCSK9 D337Y and western diet, .

Isolation of Murine Primary Aortic Smooth Muscle Cells

Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod. Myocytes, Smooth Muscle* Oligonucleotide Array Sequence Analysis.

2003;100:13531–6.However, excessive VSMC apoptosis during human and mouse aortic aneurysm development results in weakening of the aortic wall.Single-cell sequencing identified 3 unique smooth muscle cell populations in the aortic wall, 2 of which specifically localize to the neointima in a genetic disease model.In vitro studies of phosphate-induced calcification show that vascular smooth muscle cells undergo calcification with features similar to those observed in pathological vascular calcification in vivo, including the presence of hydroxyapatite crystals.Clustering analysis of gene expression from aortic cells identified 10 populations of cells representing each of the main arterial cell types: fibroblasts, vascular smooth muscle cells, endothelial cells (ECs), and immune cells, including monocytes, macrophages, and lymphocytes.

Mouse aortic biomechanics are affected by short-term defective

Auteur : Max Ole Hubert, Juan Rodriguez-Vita, Lena Wiedmann, Andreas Fischer, Andreas Fischer, Andreas Fische. Aortic aneurysm lesions (right) are characterized by thrombi, infiltration of inflammatory cells (neutrophils, macrophages, B and T cells), degradation of the ECM, VSMC phenotypic switching and apoptosis, excessive production of cytokines .Mouse aortic smooth muscle cells (SMCs) were loaded for 72 h with cholesterol by using cholesterol:methyl-β-cyclodextrin complexes, leading to ≈2-fold and ≈10-fold increases in the contents of total cholesterol and cholesteryl ester, respectively.Primary culture of vascular smooth muscle cells (VSMCs).01) formed by calcifying mouse aortic vascular smooth muscle cells (MOVAS). VSMC contractility was assessed by traction force microscopy (TFM; single cell) and collagen .Abdominal aortic aneurysm shares many features with thoracic aortic aneu-rysm, including loss of vascular smooth muscle cells (VSMCs), extracellular matrix degradation and .Mice, Inbred C57BL. Therefore, a primary culture growth protocol of cardiovascular smooth muscle cells (VSMCs) obtained from human abdominal aortas was standardized. The clonally expanded VSMCs undergo phenotypic switching towards phagocytic-like phenotypes.The aim of the present study was to observe the expression and role of NRG-1 underlying the inhibitory effect of Ang II on apoptosis of mouse aortic smooth muscle cells (MASMCs).Vascular smooth muscle cells (VSMCs) and extracellular matrix (ECM) maintain integrity of the aortic wall.

Rong JX, Shapiro M, Trogan E, Fisher EA.Thus, to better understand region and sex differences in CVD presentation, we have focused on characterizing in vitro behaviors of primary vascular smooth muscle cells (VSMCs) from the thoracic and abdominal aorta of male and female mice.

Phosphodiesterase 4D contributes to angiotensin II-induced

Bone morphogenetic proteins (BMPs) have been implicated as important .Analyses of G-protein-mediated contraction and relaxation of vascular smooth muscle cells (VSMCs) are usually hampered by a rigid growth surface and culture conditions promoting cell proliferation and a less contractile phenotype. (a) Groups of VSMCs after 2 days of culture. Macrophage-derived netrin-1 promotes abdominal aortic aneurysm formation by activating MMP3 in vascular smooth muscle cells.Transdifferentiation of mouse aortic smooth muscle cells to a macrophage-like state after cholesterol loading.Smooth muscle cell contraction is necessary to induce de-stiffening (increased compliance) after a period of high pulse pressure. BAG3 gene was specifically knockout in smooth muscle of mice and the .Thoracic aortic dissection (TAD) is a severe and extremely dangerous cardiovascular disease. scrambled HABP PAM, p≤ 0. Mouse aortic smooth muscle cells (SMCs) were loaded for 72 h with cholesterol by using cholesterol:methyl-beta-cyclodextrin complexes, leading to . The advantage of this method is that there are many more .Cell Death Discovery - Pharmacological inhibition of MALT1 (mucosa-associated lymphoid tissue lymphoma translocation protein 1) induces ferroptosis in vascular smooth muscle cells Skip to main content2-hydroxypropyl-beta-cyclodextrin (HPβCD) inhibits abdominal aortic aneurysm (AAA) formation in a vascular smooth muscle cell (VSMC) TFEB-dependent manner.Using Gsdmd-/-ApoE-/-mouse and AAV-F4/80-shGSDMD, we demonstrated the potential role of macrophage-derived GSDMD in AAA and aortic pyroptosis induced by Ang II in vivo. In the study of .Rat aortic smooth muscle cells (SMCs) were purchased from ScienCell (San Diego, California, US, .Development of the smooth muscle foam cell: uptake of macrophage lipid inclusions. Classification. Here, we describe the double-collagenase digestion method for isolating vascular smooth muscle .We describe here a technique for isolating smooth muscle cells from a single mouse aorta.

Mouse-to-mouse variation in maturation heterogeneity of smooth muscle cells

(b) A group of adherent VSMCs after 1 week of culture. Apoptosis was evaluated via TUNEL assay, MOVAS cell migration ability was assessed by monolayer wound healing assay and the levels of c‑Jun/AP‑1 and Chop were determined by western blotting, immunofluorescence and immunocytochemical assays. Heart; Aorta; Smooth . Foam-cell formation was demonstrated by accumulation of intracellular, Oil Red O .Primary cell cultures are essential tools for elucidating the physiopathological mechanisms of the cardiovascular system.We observed neogenesis of endothelial cells, smooth muscle cells, and elastic fibers from the native aorta along the Biotube at 6 months. The most significant cellular heterogeneity was seen in the 3 distinct EC . However, the characterization of SMCs at the single-cell level is stymied by drawbacks of contemporary single-cell protein measurements, including antibody probe cross-reactivity, chemical fixation artifacts, .Smooth muscle cells (SMCs) are the most abundant cells in human atherosclerotic lesions and are suggested to contribute at least 50% of atheroma foam .

20 VSMC apoptosis could be induced by excessive ROS, .Phenotypic switching in vascular smooth muscle cells (VSMCs) has been linked to aortic aneurysm, but the phenotypic landscape in aortic aneurysm is poorly .In the study of vascular biology, analyses of endothelial cells (EC) and smooth muscle cells (SMC) are very important. Moreover, HA PAMs successfully detected calcifications in atherosclerotic mouse models as well as in patient-derived .The physiology of vascular smooth muscle (VSMC) cells is affected by autophagy, a catabolic cellular mechanism responsible for nutrient recycling.In this study, RNAi screening in human aortic smooth muscle cells was performed to explore the effects of OTU family deubiquitinases, which revealed that . Proc Natl Acad Sci USA.001; HA PAM vs. 9 , 5022 (2018). non-targeting micelle, p≤ 0. Proliferation, migration and phenotypic switching of vascular smooth muscle cells (SMCs) are major pathogenetic mechanisms involved in the development of TAD.orgRecommandé pour vous en fonction de ce qui est populaire • Avis

Isolation of Murine Primary Aortic Smooth Muscle Cells

Vascular smooth muscle cells (VSMCs) of the aortic media undergo clonal expansion in mouse models of dissecting aortic aneurysms.govRole of Vascular Smooth Muscle Cell Phenotypic Switching . The absence of elastin alters the . Outlined in this paper is a step-by-step procedure for isolating aortic .