Pqe30 vector map

Paul

May 16, 2025

7 min read

Image: Illustrated plasmid map in PNG format.Plasmid pQE-30-RexYFP from Dr.

The pQE vector could carry 6 X His tags on the N or C ends of the recombinant protein. Vector Database.

SnapGene File: Plasmid sequence and SnapGene enhanced annotations.7k Plasmids: Qiagen Vectors | More Plasmid Sets.Balises :Pqe-30QiagenCloningPqe30 Expression VectorPqe Vector Set

pQE-30 vector map and sequence

Linearized bacterial expression and 6xHis-tagging vector with 3'-U overhangs for UA cloning of a PCR product. Structural Genomics Vectors. This plasmid is available through Addgene. Download Plasmid.Here, we first designed and constructed a novel pair of bacterial expression vectors, i. Fluorescent Protein Genes & Plasmids. The Dotmatics digital science platform provides the first true end-to-end solution for scientific R&D, combining an enterprise data platform . Bacterial vector for .Balises :Pqe-30QiagenSequencePlasmidCloning Vsevolod Belousov's lab contains the insert RexYFP and is published in Biochim Biophys Acta.Image: Illustrated plasmid map in PNG format.Balises :Pqe-30Polymerase chain reactionPqe Vector SetEscherichia coli For other reading frames, use pET-32a (+) or pET-32c (+). Bacterial vector for expressing C-terminally 6xHis-tagged proteins, with an NcoI site at the start codon. Growth Strain (s) DH5alpha.N-Terminus pQE Vector set (pQE-9) QIAexpress® Type IV Kit (pQE-30)*.

Preliminary studies can be carried out in bacterial expression systems, using the strong T5 promoter, which is recognized by E.Store the plasmid at -20 ℃.The pQE-TriSystem vector contains CAG, T5, and p10 promoters that enable 6xHis-tagged protein expression in mammalian, E.

N-Terminus pQE Vector Set

The C-Terminus pQE Vector Set is intended for molecular biology applications. Experiment Configurator; Discovery & Translational Research. Explore Over 2.

Bacterial vector for expression of N-terminally T7-tagged proteins.Balises :Pqe-30QiagenSequenceVector mapPlasmidVector Set (His·Strep 1) His·Strep pQE TriSystem Vector Set (His·Strep 2) Expression systems E. coli extract x x x x x His tags N-Terminal His Tag x C-Terminal His Tag x x x Number of His residues 6 6 8 8 8 Encodes .Map of pQE30, 31, 32 with multiple cloning sites. Source publication. Cell-Free DNA; DNA Clean Up; Genomic DNA; Microbial DNA; Plasmid DNA pQE-30 Xa Sequence.

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Overview of QIAGEN’s pQE Vectors

Gain unparalleled visibility of your plasmids, DNA and protein sequences. DNA & RNA Purification.pQE-30 UA Vector Positions of elements in bases Vector size (bp) 3504 Start of numbering at XhoI (CTCGAG) 1-6 T5 promoter/lac operator element 7-87 T5 transcription start 61 6xHis tag coding sequence 127-144 Multiple cloning site I 145-173 Cloning site for PCR product 174-175 Multiple cloning site II 176-235 Lambda t0 transcriptional termination region 251 .

pQE-30 Xa

pQE-30, pQE-31, and pQE-32 provide the multiple cloning site (MCS) in all three reading .

pQE-30 Sequence and Map

Source: Qiagen [9]. Growth Temperature.Download scientific diagram | Map of the pQE30, pAS01 and pAS02 vectors used in the study. The resultant vector, pAS01 (2135 bp), carries p15A replication origin and the chloramphenicol resistance gene (CmR) of pZA31MCS and T5 promoter, synthetic .Balises :Pqe-30QiagenSequenceVector mapPlasmid

pQE-30 Xa Vector

Therefore, these genes need to be precisely excised out of the pUC19 vector with restriction enzymes and ligated individually into expression vectors such as pQE30, pQE31 and pQE32 ( Figure 6) and . This product is not intended for the diagnosis, prevention, or treatment of a disease. PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.

pET-30a(+) Sequence and Map

Home Plasmids Qiagen VectorspQE-30 UA (linearized) Sticky ends from different BbsI sites may not be compatible.(EN) - C-Terminus pQE Vector Map (pQE-60) Print Bookmark Share For the pQE-60 vector pdf 48KB English Format File size Language Download Get Adobe Reader Contact QIAGEN . Bacterial vector for expressing N-terminally 6xHis-tagged proteins. Annotate features on your plasmids using the curated feature database. LOCUS Exported 3509 bp ds-DNA circular SYN 09-JAN-2013 .Balises :ColE1Pqe30Ali Samy Abdelaal, Syed Shams Yazdani

Overview of QIAGEN’s pQE Vectors

Culture cells containing pQE30::gene of no interest (GOnI) 2. Sticky ends from different BglI sites may not be compatible. pQE-30 Xa Multiple cloning site . For other reading frames, use pQE-31 or pQE-32.

(EN)

For other reading frames, use pQE-30 or pQE-32. (pQE-80L-Kan) TAGZyme pQE . Global contacts. Use with SnapGene software or the free Viewer to visualize additional data and align other sequences.Balises :Pqe-30QiagenVector mapPlasmidDNA

Bacterial expression and 6xHis-tagging vector for UA cloning of a PCR product.Balises :QiagenDNATranslational researchPqe-TrisystemPqe Vector

pQE-60 Sequence and Map

Balises :Pqe-30VectorAddgeneDatabase Bacterial vector for expressing N-terminally 6xHis-tagged proteins with a Factor Xa cleavage site. Molecular Biology Cloning of Mycobacterium smegmatis Exochelin MS genes fxbA, fxbC . Sequence Author: MilliporeSigma (Novagen) Open in SnapGene. Close the tube and incubate for 10 minutes at room temperature.Balises :PlasmidMapPqe30DiagramPlasmid pQE30-His-T7RNAP from Dr. N-Terminus pQE Vector set (pQE-40) Cis-repressed pQE Kan Vector Set. 24/7 automatic processing of online orders.Plasmid pQE30-butelase1 from Dr. Sequence Author: .pQE-30, pQE-31, and pQE-32 Vectors. SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files. GenBank File: Plasmid sequence and annotations.Positions of elements in bases.Balises :Pqe-30Vector mapDNAPqe30 SequencePqe 30 Vector For other reading frames, use pET-23b(+) or pET-23c(+). An open portfolio of interoperable, industry leading products. coli polymerase, and . Log in To see your account pricing. Growth Strain (s) NEB Stable ; JM109. Explore the Scientific R&D Platform . coli x x x x x Baculovirus-infected cells x x x Mammalian cells x x x Cell-free in EasyXpress Insect II extract Cell-free in EasyXpress E.25 µg each: pQE-16, pQE-60, pQE-70.This set provides 5 vectors (pQE-9, pQE-30, pQE-31, pQE-32, and pQE-40) for expression of N-terminally His-tagged proteins.Balises :AddgenePqe30 Expression VectorPqe30-His-CKMPqe30 Plasmid BbsI gradually loses activity when stored at -20°C. Sequence Author: Qiagen.Bacterial vector for expressing DHFR fusion proteins with an N-terminal 6xHis tag. Efficient cleavage requires at least two copies of the NmeAIII recognition sequence. This vector is NOT available from Addgene and the database is no longer actively maintained.

Download Plasmid | Download SnapGene Viewer.Welcome to Vector Database! Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

pQE-30, pQE-31, and pQE-32 Vectors

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pQE-30 Xa Sequence and Map

pQE-30 UA Sequence and Map.

pET-23a(+) Sequence and Map

Plasmid: pQE-30 XA.pET-32b (+) Bacterial vector for expressing thioredoxin fusion proteins with an enterokinase site. The maps, notes, and annotations in the zip file on this page are copyrighted material. Vector size (bp) Start of numbering at Xh oI (CTCGAG) T5 promoter/lac .pQE-30 Sequence and Map. Copy order details.Balises :Pqe-30SequenceVector map Carefully open the tube and add 20 μl of sterile water to dissolve the DNA. Welcome to Vector Database! Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources. Plasmid: pQE-30 XA. ApaI can be used between 25°C and 37°C.Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources. SnapGene File: Plasmid sequence and SnapGene enhanced . Yeast Plasmids.Provide pQE-30 Xa vector/plasmid map, full length sequence, antibiotic resistance, size and other information. Sticky ends from different EcoO109I sites may not be compatible. Search vector database. 3461 1–6 7–87. Open in SnapGene. Try SnapGene for . The pQE-TriSystem Vector is intended for molecular . Cell-Free DNA; DNA Clean Up; Genomic DNA; Microbial DNA; .SnapGene Viewer.pQE-30 Xa Vector Positions of elements in bases Vector size (bp) 3509 Start of numbering at XhoI (CTCGAG) 1-6 T5 promoter/lac operator element 7-87 T5 transcription start 61 . Bacterial Expression. Mammalian Expression Vectors. Takuya Ueda's lab contains the insert T7 RNA polymerase and is published in Nat Biotechnol. 2001 Aug;19(8):751-5. Plan your experiment; Discovery & Translational Research. Try SnapGene for Free. Use text editor or plasmid mapping software to view sequence.File size Language Download Get Adobe Reader Bacterial Resistance (s) Ampicillin, 100 μg/mL. Plasmid: pET-30 a (+)Bacterial vector for ligation-independent cloning (LIC) to express 6xHis- and S-tagged proteins with an enterokinase site. coli, and baculovirus-infected insect cells, respectively (see figure pQE TriSystem).pQE-30 Xa Sequence and Map. Cloning Method. Midi Prep Purification (2 preps) Protocol: Plasmid or Cosmid DNA Purification Using HiSpeed Plasmid Midi and Maxi Kits This protocol is for preparation of up to 200 μg of high-copy or low-copy plasmid or cosmid DNA using the HiSpeed Plasmid Midi Kit or 750 μg using the HiSpeed Plasmid .pQE-31 Sequence and Map.Show Static Map

pQE-30 UA Sequence and Map

The pAS01 was obtained by combining portion of the pZA31MCS vector and the pQE30 vector together.