Qiaquick spin columns

Nancy Walker

Jul 1, 2025

8 min read

DNA of up to 10 kb is .QIAquick Spin Columns 50 250 Buffer PB* 30 ml 150 ml Buffer PE (concentrate) 2 x 6 ml 55 ml Buffer EB 15 ml 55 ml pH Indicator I 800 µl 800 µl Collection . To wash, add 0.QIAquick Nucleotide Removal Kit提供离心柱、缓冲液和收集管，基于硅胶膜技术，纯化寡核苷酸和DNA。去除没有整合的核苷酸、盐和其他杂质。使用快速的结合、洗涤、洗脱过程使用30–50 μl洗脱体积即可纯化大于17 nt的寡核苷酸和40 bp–10 kb的DNA。该流程可在QIAcube全自动核酸纯化仪上全自动运行。 NOTE: Due to special . Filter paper discs were punched from sheets of Whatman . 28115) in the QIAquick PCR Purification, Gel Extraction, Nucleotide Removal and PCR & Gel Cleanup kits are also sold separately from the kits.

Gel loading dye for convenient sample analysis. Discard flow-through and place the spin .Balises :Spin ColumnsQIAquick PCR PurificationDNA10 µgBrand:Qiagen The spin columns are designed to allow elution in very small volumes (as little as 10 µL), delivering high yields of highly concentrated DNA.Silica columns from PCR purification and gel extraction kits are widely used in laboratories worldwide to assist in gene cloning. color of the mixture will turn to yellow. 3 Place a QIAquick spin column in a provided 2 ml .QIAquick Spin Columns (100) PRODUCT AVAILABILITY: Did you know you can view a product's availability right on the product page? Simply enter the quantity you want to purchase and the current availability will appear below the item.The QIAquick PCR Purification Kit provides spin columns, buffers, and collection tubes for silica membrane based purification of PCR products >100 bp.

QIAquick Spin Handbook

Using our spin columns as an OEM component will help you ease the regulatory compliance burden while our global reach can simplify your logistics . In addition, the binding capacity and DNA recovery size cut-offs of the QIAprep and QIAquick Spin columns are different . The MinElute Gel Extraction Kit provides spin columns, buffers, and collection tubes for silica-membrane-based purification of DNA fragments of 70 bp – 4 kb from up to 400 mg gel slices.QIAquick spin columns offer three handling options: aside from the usual microcentrifuge, they can now also be used in any commercial vacuum manifold with luer connectors (e.

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Place a QIAquick spin column in a provided 2 ml collection tube. Collectiontubesarere-usedtoreduceplasticwaste. Efficiency of SYBR Green dye removal has to be validated by the enduser.Taille du fichier : 714KB

November 2018 QIAquick Spin Handbook

To bind DNA, apply the sample to the QIAquick column and centrifuge for 30–60 s. To recharge spin columns with this format, we loaded one or two layers of filter paper discs into the column by pushing the discs into the bottom using the end of a 200 μl pipette tip .color of the mixture is orange or violet, add 10 µl o f 3 M s odium acetate, pH 5. DNA of up to 10 kb is purified using a simple and fast bind-wash-elute procedure using a microcentrifuge or vacuum manifold and an elution volume of 30–50 μl.Balises :QIAquick PCR PurificationDNAQiaquick Gel ExtractionBalises :Spin ColumnsQIAquick PCR PurificationDNA10 µgBrand:Qiaquick

Rapid Regeneration and Reuse of Silica Columns from PCR

QIAquick Spin columns: 50: 250: Buffer PB* 30 ml: 150ml: Buffer PE (concentrate) 2 x 6 ml: 55 ml: Buffer EB: 15 ml: 55 ml: pH Indicator I: 800 µl: 800 µl: Collection Tubes (2 ml) 50: 250: Loading Dye: 100 µl: 550 µl * Buffers PB, PNI and QG contain chaotropic salts, which are irritants.Balises :Extraction of SilicaPublish Year:2018 While columns from the QIAprep Spin Miniprep Kit and the QIAquick PCR Purification- and Gel Extraction Kits are based on silica-gel-membrane technology, each is designed to work optimally within its own kit format.

Are QIAprep and QIAquick Spin columns interchangeable?

The QIAquick PCR Purification Kit provides spin columns, buffers, and collection tubes for silica-membrane-based purification of PCR products >100 bp.5) to the center of the QIAquick membrane and centrifuge the column for 1 min at 13,000 rpm ( 17,900 xg).Balises :Spin ColumnsDNA

28506-QIAquick Spin Columns (100)- Qiagen » EAD SCIENTIFIC

Balises :Spin ColumnsQIAquick PCR PurificationExtraction of SilicaThe QIAquick Gel Extraction Kit provides spin columns, buffers, and collection tubes for silica-membrane-based purification of DNA fragments from gels (up to 400 mg slices) or enzymatic reactions.QIAquick® Spin Columns can now be used on any vacuum manifold with luer connectors, for example, the QIAvac 6S or QIAvac 24 with QIAvac Luer Adapters.Balises :Spin Columns10 µgQIAquick Gel Extraction KitQiagen Dna Extraction KitPlace the QIAquick column in a clean 1. The MinElute columns in the MinElute PCR Purification, Gel Extraction and Reaction Cleanup kits are not sold separately.Place a QIAquick spin column in a provided 2 ml tube. Place the QIAquick column back into the same tube.

QIAquick Gel Extraction Kit

However, the use of these columns .

Then, 100 ng amplified library were .Simple – GeneJET kits feature trusted silica membrane technology in the form of a simple and convenient spin column for purification of DNA and RNA.QIAquick Spin Handbook - Kirschner Labkirschner.4 QIAquick Spinプロトコールとトラブルシューティング03/2008 6.5) to the center of .

QIAquick spin columns offer three handling options: aside from the usual microcentrifuge, they can now also be used in any commercial vacuum manifold with luer connectors . 洗浄のために750 µlのBuffer PEをQIAquickカラムにアプライし、30～60秒間 遠心操作する。 7.

MinElute PCR Purification Kit

However, the use of these columns can generate plastic waste that . Discard flow-through.QIAquick PCR Purification Kit (250) For purification of 250 PCR reactions: 250 QIAquick Spin Columns, Buffers, Collection Tubes (2 ml) 28106. We always provide extra buffers in our .Balises :Spin ColumnsBrand:Qiagen QIAquick Spin columns (QIAGEN 28115)纯化多至10 μg PCR产物，100 ., QIAvac 24 Plus with QIAvac Luer Adapters) or undergo automated processing in QIAcube Connect (see page 10).The preferred spin column format has a flat bottom with a net-like structure to support binding matrix . The QIAquick Gel Extraction Kit, in addition to other QIAGEN spin-column-based kits, can .5) or water (pH 7.単品販売のQIAquick Spin Columns (100)と組み合わせて使用すると経済的です。 製品名 内容 Cat.Cleanup of DNA up to 10 kb in three easy steps.75 ml Buffer PE to the QIAquick column .

MinElute Reaction Cleanup Kit

Qiagen QIAquick PCR Purification Kit Protocol v1

This protocol is designed for removal of primers 17 nucleotides.comRecommandé pour vous en fonction de ce qui est populaire • Avis

QIAquick Spin Handbook

Adsorption to QIAquick membrane — salt and pH dependence .Balises :DNAFormat:Spin columns24 minipreps in 30 minutes1-24 samples

To elute DNA, add 100–200 µl of Buffer EB (10 mM Tris·Cl, pH 8.Product Details.

QIAprep Spin Miniprep Columns

Place QIAquick column in a clean 1.QIAquick Spin Handbook - QIAGENqiagen. Universal – Purified nucleic acids are . QIAquick Spin Handbook - (EN) Print Bookmark Share.The wide range of reagents are suitable for use with nucleic acids in transfection and transformation procedures, as well as cloning, sequencing, purification, and extraction.

28115-QIAquick Spin Columns (100)- Qiagen » EAD SCIENTIFIC

To elute DNA, add 50 μl Buffer EB (10 mM Tris·Cl, pH 8.QIAquick Spin Handbook 03/2008. Take appropriate laboratory safety measures and wear gloves when .< 40mersBalises :Spin ColumnsQIAquick Gel Extraction KitQiagen Dna Extraction KitWe strongly assume that the QIAquick Gel Extraction Kit will be equally efficient in removing this dye, however, we recommend a 5 min incubation with wash Buffer PE on the QIAquick spin column at step 10 of the QIAquick Gel Extraction Kit Protocol.QIAquick Spin Handbook - QIAGEN.5) to the center of the QIAquick membrane and centrifuge the column for 1 min. They are compatible with QIAvac 24 Plus and TRACKMAN .QIAquick Spin Columns are silica-membrane-based spin columns for removing nucleotides, enzymes, salts, and other impurities from DNA samples.The reactions were pooled over two QIAquick Spin Columns (Qiagen, manufacturer’s protocol) and agarose gel purified using three QIAquick Spin Columns. The QIAprep Spin Miniprep Kit enables purification of up to 20 μg molecular biology grade plasmid DNA or cosmid DNA for use in routine molecular biology .

Rapid Regeneration and Reuse of Silica Columns from PCR

Alternatively, for increased DNA concentration, add 30 μl elution buffer to the .QIAquick Spin Columns 50 250 Buffer QG* 2 x 50 ml 2 x 250 ml Buffer PE (concentrate) 2 x 10 ml 2 x 50 ml Buffer EB 15 ml 15 ml Collection Tubes (2 ml) 50 250 Handbook 1 1 * . An optional pH indicator allows easy .QIAquick Spin columns. The spin columns fit into a conventional table-top microcentrifuge or onto any vacuum manifold with luer connectors, such as the QIAvac 24 Plus with QIAvac Luer Adapters.00 | 品牌：QIAGEN. 货号：28115 | 规格：100 Reactions | 价格：￥0.5 ml microcentrifuge tube.This is performed with high concentration of EDTA solution for demineralization of bone powder followed by QIAamp ® spin columns and buffers from the QIAquick ® . Binding capacity of 10 µg.Balises :QIAquick PCR PurificationLaboratory Techniques in BiologyFor gel extraction or cleanup of 1000 reactions: 1000 QIAquick Spin Columns, Buffers, Collection Tubes (2 ml) 28706X4: 询价 QIAquick PCR & Gel Cleanup Kit (100) For purification PCR fragments and gel extractions of 100 reactions (at least 80–100 gel reactions or 100 PCRs) 28506 : 询价 .Shop Qiagen QIAquick Spin Columns (100) at Thomas Scientific, your trusted partner in Science. To bind DNA, apply the sample to the QIAquick column, centrifuge for 1 min.QIAquick Spin Columns are silica-membrane-based spin columns for purification of PCR products >100 bp. QIAquick PCR Purification Kitは分子生物学的アプリケーション用であり、疾病の診断、予防、あるいは治療に使用することは .Marque : Qiagen

QIAquick Spin Handbook

DNA ranging from 70 bp to 10 kb is purified using a simple and fast bind-wash-elute procedure and an elution volume of 30–50 μl.